Haplotype Analysis of Apoa2 Region in Mice with APOA2b
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Strains with APOA2b |
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| Marker1 |
Gene2 |
Start (bp)3 |
End (bp)3 |
129 |
BALB |
C3H |
CBA |
FVB |
KK |
LP |
NON |
NZB |
NZW |
RF |
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| Wrn3 | 175048841 | 175048962 | 2 | 2 | 2 | 1 | 2 | 3 | 3 | 2 | 2 | 1 | 1 | |||||||||||
| Nr1i3 | 175071723 | 175076568 | ||||||||||||||||||||||
| Wrn1 | 175081943 | 175082139 | 2 | 1 | 2 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | |||||||||||
| Apoa2 | 175082812 | 175084093 | ||||||||||||||||||||||
| Fcer1g | 175087300 | 175092009 | ||||||||||||||||||||||
| Wrs1 | 175092624 | 175092806 | 2 | 2 | 1 | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 2 | |||||||||||
| Ndufs2 | 175092583 | 175104835 | ||||||||||||||||||||||
| Wrs31
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175106847
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175107095
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3
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3
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3
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3
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2
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1
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3
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1
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2
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1
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3
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↵1 Microsatellite markers that we designed. We used PCR to amplify them. PCR products were scored according their sizes, with 1 < 2 < 3 in size. The sequences of the PCR primers for these markers are: Wrn3: forward: 5′-CCCAAAGGATTTACACATGC-3′, reverse: 5′-TACATACACCTGCCACATGC-3′; Wrn1: forward: 5′-TGCAGCATTTTCTCTGTGTG-3′, reverse: 5′-AAGGAATGGGGGTTATGAAG-3′; Wrs1: forward: 5′-CTAGTTCATGCACAGAAAGCC-3′, reverse: 5′-CCAGGATATTGTGTTCGGAG-3′; Wrs3: forward: 5′-AGTTCCCTCCTCTAACACCC-3′, reverse: 5′-CACCCAGAAGTCATCTCTGC-3′.
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↵2 Genes that are closest to Apoa2, according to Ensembl Mouse Genome Server (http://www.ensembl.org/Mus_musculus/), Build 32.
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↵3 Positions were retrieved from Ensembl Mouse Genome Server (http://www.ensembl.org/Mus_musculus), Build 32. The distance from the neighboring genes to Apoa2: Nr1i3: 6244 bp; Fcer1g: 3207 bp; Nudfs2: 8490 bp.
