Table 1.
Sequence of Synthetic Oligos and Extension Primers
|
Synthetic oligos as extension templates |
Extension primers |
Misincorporation |
|---|---|---|
| Template 1 (G/C) | ||
| GTGGTGTTCCTCTTCGAAGGGCTTGCTAATCCTTGGCCCA | CCAAGGATTAGCAAGCCCTTCGAAGAG | G allele for CC cluster |
| GTGGTGTTGCTCTTCGAAGGGCTTGCTAATCCTTGGCCCA | ||
| Template 2 (G/A) | ||
| AGTGGATCCCACTGTCGATGGAGATGCCTGAGAAAGACCC | CTCAGGCATCTCCATCGACAGTGGG | G allele for AA cluster |
| AGTGGACCCCACTGTCGATGGAGATGCCTGAGAAAGACCC | CTCAGGCATCTCCATCGACAGTGGG | |
| Template 3 (A/T) | ||
| ACGAAAATTTTGCTAGTGGTTTACCCATGGTCATAGCTGT | CTATGACCATGGGTAAACCACTAGCAAAA | A allele for TT cluster |
| ACGAAATTTTTGCTAGTGGTTTACCCATGGTCATAGCTGT | ||
| Template 4 (G/A) | ||
| CAAAACTAAGATAGCTATTCCATCCCTCCATTCGTTTTTATTATC | AACGAATGGAGGGATGGAATA | A allele for GG cluster |
| CAAAACTAAGATAGTTATTCCATCCCTCCATTCGTTTTTATTATC | ||
| Template 5 (A/T) | ||
| CACTAGCAAAAATTTCGTGTCCTGTTCCCACTGGCCGTCG | GGCCAGTGGGAACAGGACACGAAA | A allele for TT cluster |
| CACTAGCAAAATTTTCGTGTCCTGTTCCCACTGGCCGTCG | ||
| Template 6 (G/A) | ||
| AGTGGATCCCACTGTCGATGGAGATGCCTGAGAAAGACCC | CTCAGGCATCTCCATCGACAGTGGG | G allele for AA cluster |
| AGTGGACCCCACTGTCGATGGAGATGCCTGAGAAAGACCC
|
|
|
