Role of Excess Inorganic Pyrophosphate in Primer-Extension Genotyping Assays

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 6
Figure 6

Results of genotyping assay using the Texas red/TAMRA acycloterminator combination. (A) Genotyping results of rs183702 on 48 DNA samples using the TAMRA/R110 combination. The G/A heterozygous and G/G homozygous clusters collapsed into A/A homozygous and blanks, respectively, because there were no difference in FP value between free R110 acycloterminators and primer extension products. (B) Genotyping results of rs183702 using the Texas red/TAMRA acycloterminator combination. Four clusters are clearly seen.

This Article

  1. Published in Advance August 12, 2004, doi: 10.1101/gr.2833204 Genome Res. 14: 1749-1755

Preprint Server



Current Issue

  1. March 2026, 36 (3)
  1. Current Issue
  1. Alert me to new issues of Genome Research