Role of Excess Inorganic Pyrophosphate in Primer-Extension Genotyping Assays

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Figure 4
Figure 4

Combining FP and intensity ratio (quenching) in data analysis. (A) Genotyping results of rs4723564 when the data were plotted as TAMRA FP versus R110 FP. Low FP values of R110 resulted in poor separation of clusters. (B) Same data were replotted by using TAMRA FP values versus the intensity ratio R. Four clear clusters were formed when quenching was taken into account.

This Article

  1. Published in Advance August 12, 2004, doi: 10.1101/gr.2833204 Genome Res. 14: 1749-1755

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