List of Experimentally Detected or Cloned miRNAs at the Mouse Distal 12 Domain
|
miRNAa |
Sequence (5′ to 3′)b |
Maternally expressed miRNAs |
References |
|---|---|---|---|
| miR-342 | UCUCACACAGAAAUCGCACCCGUC | n.d. | Kim et al. 2004; this study |
| miR-345 | UGCUGACCCCUAGUCCAGUGC | — | Kim et al. 2004; this study |
| miR-127 | UCGGAUCCGUCUGAGCUUGGCU | + | Lagos-Quintana et al. 2002; Seitz et al. 2003 |
| miR-136 | ACUCCAUUUGUUUUGAUGAUGGA | + | Lagos-Quintana et al. 2002; Seitz et al. 2003 |
| miR-134 | UGUGACUGGUUGACCAGAGGG | + | Lagos-Quintana et al. 2002; this study |
| miR-154/miR-A19-5c | UAGGUUAUCCGUGUUGCCUUCG | + | Lagos-Quintana et al. 2002; this study |
| miR-323/miR-A1-3′ | GCACAUUACACGGUCGACCUCU | n.d. | Kim et al. 2004; this study |
| miR-329/miR-A2-3′ | AACACACCCAGCUAACCUUUUU | n.d. | Kim et al. 2004; this study |
| miR-300/miR-A9-3′ | UAUGCAAGGGCAAGCUCUCUUC | n.d. | Houbaviy et al. 2003; this study |
| miR-409/miR-A22-3′ | GAAUGUUGCUCGGUGAACCCCUU | n.d. | this study |
| miR-410/miR-A24-3′ | AAUAUAACACAGAUGGCCUGUU | + | this study |
| miR-376b/miR-B4 | AUCAUAGAGGAACAUCCACUUU | + | this study |
| miR-376/miR-B6 | AUCGUAGAGGAAAAUCCACGUU | + | this study |
| miR-411/miR-C2 | AACACGGUCCACUAACCCUCAGU | + | this study |
| miR-380-3p/miR-C3 | UAUGUAGUAUGGUCCACAUCUU | + | this study |
| miR-299/miR-D | UGGUUUACCGUCCCACAUACAU | n.d. | Houbaviy et al. 2003; this study |
| miR-412/miR-K | ACUUCACCUGGUCCACUAGCCGU | n.d. | this study |
| miR-337/miR-M | UCAGCUCCUAUAUGAUGCCUUUC | + | Kim et al. 2004; this study |
| miR-370/miR-Nd | GCCUGCUGGGGUGGAACCUGGUU | + | this study |
| miR-341e
|
UCGAUCGGUCGGUCGGUCAGU
|
n.d.
|
Kim et al. 2004
|
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n.d. indicates not determined.
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↵a Novel miRNA sequences have been submitted to the miRNA registry (http://www.sanger.ac.uk/cgi-bin/Rfam/mirna/browse.pl.) and they have been given new names in accordance to the miRNA registry numbering (e.g. miR-A22-3′ is also called miR-409 in the miRNA registry).
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↵b The exact length of the miRNAs which have been detected solely by primer extension is not known. Thus, the two last nucleotides at the 3′-termini are in italics as they are only predicted based on a 23 nt theoretical long RNA species. Primer extension assay generates two cDNA products with a size differing by one nucleotide. Based on the miR-134 and miR-154 sequences obtained independently by cloning strategies (Lagos-Quintana et al. 2002), we have considered the shorter cDNA product as the correct 5′ termini while the longer one might correspond to in vivo processing heterogeneity and/or addition of an extra nucleotide in a matrix-independent manner by the AMV reverse transcriptase (Promega technical service, pers. comm.).
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↵c miR-154/A19 are processed from the 5′ side of A19 (Lagos-Quintana et al. 2002) while miR-323/A1, miR-329/A2, miR-A9, miR-A22 and miR-A24 are processed from the 3′ strand of the A1, A2, A9, A22 and A24 gene copies, respectively suggesting that both strands of A-type can potentially be converted to miRNAs.
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↵d Depending upon RNA samples, we could also detect a ladder-like pattern superimposed to the mature 5′ end of miR-N. Thus, this miRNA might not fulfill the stringent criteria described in (Ambros et al. 2003).
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↵e mir-341 was not found in our in silico search as it is not conserved at the human inprinted 14q32 interval.
