Expression of in silico-predicted miRNA genes. Tissue-specific (A) and developmental gene expression pattern (B) of the miRNAs analyzed by primer extension assay. H indicates head; T, trunk. 5.8S and let-7 probes have been used as gel loading controls (Northern blot analysis). (C) The miRNA genes are only expressed from the maternally inherited chromosome. Ten micrograms of total RNA extracted from wild-type embryos, embryos with maternal uniparental disomy for chromosome 12 (matUPD12), or embryos with paternal uniparental disomy for chromosome 12 (patUPD12) have been subjected to primer extension assay. (D) The miRNA imprinted gene expression is controlled by the intergenic germline-derived differentially methylated region (IG-DMR). Ten micrograms of total RNA extracted from embryo with a deletion of the IG-DMR from either the maternally or the paternally inherited chromosomes (as specified at the top of the picture) have been subjected to primer extension analysis.
