Figure 4
Epigenetic and sequence comparison of conserved core region of DMR2 in IGF2. (A) Multispecies alignment of DMR2 core; conserved CpGs are boxed. (B) Methylation profiles of opossum and platypus CG dinucleotides from individual cloned alleles following PCR amplification of bisulfite-modified genomic DNA. Filled circles represent methylated cytosines in the context of CpG dinucleotides. The number of individual clones with each methylation profile is listed. (C) Core CpG dinucleotide methylation sequencing analysis from the opossum and platypus orthologous DMR2 region following bisulfite modification. Methylated cytosines are labeled with arrowheads.
