The Use of MPSS for Whole-Genome Transcriptional Analysis in Arabidopsis

Table 3.

Classification of 17-Base Genomic and Expressed Signatures





Secondary class






Class
Position
Arabidopsis genomea
3
6
# with hits = 1
% with hits = 1b
Grouped by genec
Mean # per gene
# in MPSS librariesd
MPSS data, grouped by gened
1 Within exon, same strand 203,174 204 149 168,024 82.7 28,134 7.22 29,352 16,312
2 Within 500 bp potential 3′UTR 44,536 7,047 733 37,831 84.9 21,735 2.05 6,844 5,862
3 Antisense to exon 197,083 163,052 82.7 27,813 7.09 16,897 9,474
4 Unannotated 288,142 223,609 77.6 5,622
5 Within intron, sense strand 60,430 153 107 53,639 88.8 17,003 3.55 1,476 1,317
6 Within intron, antisense strand 57,847 51,354 88.8 16,715 3.46 685 650
7 Spans an exon/intron splice site 6,807 5,652 83.0 5,369 1.27 955 920
Total 858,019 7,404 989 703,161 82.0 28,912 61,831 19,820
0 Non genomic match 19,970
1/2/5/7
Normal coding gene
314,947




28,911

38,627
19,088
  • a Number of signatures occurring at distinct positions in TIGR annotation version 3.0.

  • b Calculated as the number of signatures in each class with hits = 1 divided by the total number of signatures in that class.

  • c This value is the union of the set of gene identifiers matched by all genomic signatures in each class.

  • d From 14 libraries, considering only “significant” and “reliable” expressed signatures for which hits = 1, and excluding 37,192 “nonsignificant” hits to the genome. Calculated as the union of the set of all libraries.

This Article

  1. Genome Res. 14: 1641-1653

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