Features Differentiating Between Alternatively Spliced and Constitutively Spliced Exons
|
|
Alternatively spliced exons |
Constitutively spliced exons |
P-valuea |
|---|---|---|---|
| Average size | 87 | 128 | P < 10-16 |
| Percent exons whose length is a multiple of 3 | 73% (177/243) | 37% (642/1753) | P < 10-9 |
| Average human—mouse exon conservationb | 94% | 89% | P < 10-36 |
| Percent exons with upstream intronic elements conserved in mousec | 92% (223/243) | 45% (788/1753) | P < 10-11 |
| Percent exons with downstream intronic elements conserved in mousec | 82% (199/243) | 35% (611/1753) | P <10-14 |
| Percent exons with both upstream and downstream intronic elements conserved in mousec
|
77% (188/243)
|
17% (292/1753)
|
P < 10-37
|
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↵a P-value was calculated using Fisher's exact test, except for the “average size” and “average human—mouse exon conservation”, for which P-value was calculated using student's t-test.
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↵b Average percent of matching nucleotides in global alignment of the respective exons.
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↵c The 100 intronic nucleotides immediately upstream (or downstream) of the exon were locally aligned with the mouse 100 counterpart intronic nucleotides using Sim4 (Florea et al. 1998). Conservation was defined if at least 12 consecutive perfectly matching nucleotides were found in the alignment.
