Figure 5
TaqMan Real Time PCR verification performed for DOC1, KLF4, TC1, and ID1 on cDNA from human fibroblast cells treated for 30 min, 1, 2, 4, and 8 h with both antiangiogenic agents (10 μg/mL endostatin and 10 nM fumagillin) and the cytotoxic agent (50 μg/mL 5FU). No significant changes in gene expression for these four genes are detectable in fibroblasts after treatment with antiangiogenic and cytotoxic compounds. The y-axis represents a ratio between the number of ds-cDNA molecules of the gene of interest and the number of ds-cDNA molecules of the housekeeping gene GAPDH. Each dot is an average of three experiments.
