TaqMan Real Time PCR verification performed for DOC1, KLF4, TC1, and ID1 on cDNA from HUVECs treated for 30 min, 1, 2, 4, and 8 h with both antiangiogenic agents (10 μg/mL endostatin and 10 nM fumagillin) and the cytotoxic agent (50 μg/mL 5FU). DOC1, KLF4, and TC1 showed the same gene expression changes as the microarray data. ID1 was not confirmed, most likely due to cross-hybridization with some similar gene on the cDNA array. DOC1 under 5FU treatment shows a progressive increase of gene expression over the time course. The y-axis represents a ratio between the number of ds-cDNA molecules of the gene of interest and the number of ds-cDNA molecules of the housekeeping gene GAPDH. Each dot is an average of three experiments.
