Stress-Induced DNA Duplex Destabilization (SIDD) in the E. coli Genome: SIDD Sites Are Closely Associated With Promoters

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 2
Figure 2

The SIDD profile of a representative 5-kb region of the E. coli genome. ORF locations are given by the bars above the graph; the upper level of bars are ORFs that transcribe directly; the lower level are ORFs that transcribe in reverse. The documented dnaK promoters also are shown. Destabilization is largely confined to the 5′-flanks of coding regions. The strongest SIDD site is between the divergently oriented dnaK and yaaI ORFs, a region that contains both dnaK promoters. The convergent terminal region at 11,300 is destabilized below G(x) < 5, but the other such region at position 9900 is not.

This Article

  1. Genome Res. 14: 1575-1584

Preprint Server