Table 2.
The Relative Solvent Accessibility of ABPαβγ ω+ Sites That Map to the Recombinant Cat Allergen Fel DI (PDB 1PUO)
|
|
1PUO chain 1 |
ABPα |
1PUO chain 2 |
ABPβγ |
||||
|---|---|---|---|---|---|---|---|---|
|
|
All sites |
Mouse and rat ω+ |
Mouse only ω+ |
All sites |
Mouse and rat ω+ |
Mouse only ω+ |
||
| No. of residues | 68 | 4 | 5 | 67 | 29 | 17 | ||
| No. buried | 5 | 0 | 0 | 6 | 3 | 1 | ||
| No. intermediate | 22 | 0 | 0 | 19 | 6 | 2 | ||
| No. exposed | 41 | 4 | 5 | 42 | 20 | 14 | ||
| Buried (%) | 7.4 | 0.0 | 0.0 | 9.0 | 10.3 | 5.9 | ||
| Intermediate (%) | 32.4 | 0.0 | 0.0 | 28.4 | 20.7 | 11.8 | ||
| Exposed (%)
|
60.3
|
100.0
|
100.0
|
62.7
|
69.0
|
82.4
|
||
-
The solvent accessibility values of cat Fel dl residues equivalent to ABPαβγ ω+ residues were calculated from the structure coordinates using the DSSP algorithm (Kabsch and Sander 1983). Relative accessibility scores were partitioned into three states: buried (<9% relative accessibility), intermediate (9%-35% relative accessibility), and exposed (≥36% relative accessibility), as described previously (Rost and Sander 1994).
