Sequencing traces allowing detection of 1% allele. This is an example of the enrichment of 1% allele detected by the mutation surveyor software (variation 1 in Fig. 4A). The top two traces are the forward traces from the standard and the variant-enriched pool, and the bottom two traces are the reverse traces. The letters R and S in the left side of each trace denote the standard and variant-enriched pool, respectively. The middle traces are the software graphical representation of the “difference” between the traces. At the top, the sequence of the traces (uppercase letters) are aligned with the public database sequence (lowercase letters), allowing the localization of the variation.
