Figure 3
Sequencing traces assessing enrichment after one and two MRD rounds. Sequencing traces of products that had undergone zero (top), one (middle) or two (bottom) MRD rounds. The red box indicates the site of variation (C/T SNP). The reference has the C, and the variant base is the T. The bases have the following colors: A, green; G, black; T, red; and C, blue. The top trace is a sequence of the PCR amplified from 100 whites. The T/C polymorphism is evident after the first round of enrichment, and after the second round, the T becomes the only major peak. Of note is that this SNP is the same as that in Figure 1.
