An Efficient Method to Generate Chromosomal Rearrangements by Targeted DNA Double-Strand Breaks in Drosophila melanogaster

Table 4.

Frequency of Gene Targeting to a DSB

Strain	*P-element DE16* insertion**	*P-element DE20* insertion**	EGFP⁺ w⁺ y^-	w⁺ y⁺	Frequency
α	16d^a (3L;79A;mub)^b	20z (3R;100D;ttk)	12 (6)^c	2250	0.53%
β	16c (3R;83C;CG2017)	20h (3L; 62E;CG32306)	10 (5)	1300	0.77%
γ	16c (3R;86C;CG2017)	20y (3R; 86E;CG14709)	4 (3)	1050	0.38%
Total			26 (14)	4600	0.56%

↵a Letters arbitrarily denote the isolated insertion.
↵b The cytological position together with the gene next to the insertion site is given in parentheses.
↵c The minimal number of independent events is given in parentheses.

Published in Advance June 14, 2004, doi: 10.1101/gr.2279804 Genome Res. 2004. 14: 1382-1393

An Efficient Method to Generate Chromosomal Rearrangements by Targeted DNA Double-Strand Breaks in Drosophila melanogaster