Table 2.
NHEJ Events Deleting the First Exon of the Yellow Locus in DE20
|
Ea: 5′-ATTCGATAGGGTAATACTAGT-3′ |
Δ 0.88 kb, +1 bp |
| C: 5′-GATTTAACAGGGTAATACTAGT-3′ | Δ 1.3 kb |
| Y: 5′-CTAAAGTATT*ATAACAGGGTAATACTAGT-3′ | Δ 0.37 kb, +4 bp |
| H: 5′-GTATGGGTATAACAGGTATAACCCTGTACAGGTATATACCTGTACCCTGTACCCTGTACCCTACTAG-3′
|
Δ 0.88 kb, +54 bp
|
-
Events are derived from strain δϵ. Nucleotides derived from the I-Scel site are underlined and the site of cleavage is indicated with an asterisk. The size of the deletion (Δ) is given on the right. Nucleotides, which have been inserted during the process of NHEJ are indicated in bold letters, and the number is given on the right (+).
-
↵a Letters arbitrarily denote the isolated event.
