Frequency of Chromosomal Rearrangements by HR
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A. Frequency of Chromosal Inversions |
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| Strain
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P-element DE16 insertion
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P-element DE20 insertion
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Inversion size (Mbp)
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Yellow loss (w+y- / w+y- + w+y+)
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Episomal EGFP-
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Rearrangement by SSA (according to Southern)
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Inversions
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| α | 16da (3L;79A;mub)b | 20z (3R;100D;ttk) | 29 | 230/1789 (12.8%) | 31/94; 33% | 26 of 26 | 4.12% (17)c | ||
| β | 16c (3R;83C;CG2017) | 20h (3L; 62E;CG32306) | 22.6 | 40/466 (8.6%) | 9/19; 47% | 8 of 9 | 3.9% (3) | ||
| γ | 16c (3R;83C;CG2017) | 20y (3R; 86E; CG14709) | 5.7 | 27/836 (3.2%) | ND | 1 | ∼0.8% (1) | ||
| B. Frequency of Chromosomal Translocations | |||||||||
| Strain
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P-element DE18 insertion
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P-element DE20 insertion
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Yellow loss
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Other loss of y events
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Translocations
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| δϵ
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18ca (2R;45C)b
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20d (3R;89A)
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46/955 (4.8%)
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3% (8; 4 NHEJ, 4 ND)
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1.8% (5)c
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The frequency of chromosomal rearrangement is not equivalent to the number of events since the heat shock is performed at larval stages, and thus, the frequency is also influenced by the expansion of germ cells during development. Inversion frequencies for individual starter strains were calculated from the number of w+y- flies without episomal EGFP expression and corrected by a factor 0.97 because one out of 35 inversions was a false-positive event according to Southern data. For strain γ the frequency was calculated according to the Southern data only, where 25% of the w+y- had an inverted chromosome. ND indicates not determined.
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↵a Letters arbitrarily denote the isolated insertion.
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↵b The cytological position together with the gene next to the insertion site is given in parentheses.
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↵c The minimal number of independent events is given in parentheses.
