Protein Interactions Detected by Y2H Library Screens
|
Bait construct |
Library screen interactionsd |
|---|---|
| Rrp41 | PM/ScI-75 (5), FLJ20297 (3), C1or19 (3), DKFZp566J2046 (3), FLJ33418, EEF1A1, NEK1, BC006102, BM770606, AKR1A1, GTF2IRD1, LRRC5, FLJ21047, PLINP |
| Rrp42/KIAA0116 | — |
| Rrp46c | OIP2 (94), Lsm5 (8), Rrp40 (4), EBP1 (3), NUP210 (2), CsI4, PKM2, SFPQ, FLJ30932, DCK5RAP1, POLR2L |
| PM/ScI-75/Rrp45 | — |
| Mtr3 | EIF3S2 (5), Rrp42 (5), GRIM19 (2), SF2p32, SFRS3, CPSF5, RAB3-GAP150, PSMB1, ERRalpha, AD023, MOCS3, FLJ22678, WBSCR18, KIAA0182 |
| Oip2 | FHOD1 (11), RASSF1 (9), MKRN1 (8), KIAA1604, AI685517, FOXN3 |
| Rrp4c | Rrp42 (49), Znf408 (6), RNF8 (2), FLJ40491 |
| Rrp40 | Rrp46, CDK5RAP1 |
| CsI4 | NUP160 (17), Rrp41 (3), AV753896 |
| PM/ScI-100/Rrp6 | CIB1 (3), C14orf92, SCRIB, PTGES2, BM451300, |
| exonuclease [1-456] | PM5 (2), GA17, FACT-80, USP16, EPPB9, H17, FLJ22678, FLJ23784, AL110135, AL512687 |
| HRDC [503-583] | FLJ11934 (2), TARDBP, RPE, URP2, |
| [584-860] | RUVBL2, AJ431196, AI620703 |
| Kiaa0052b | — |
| Mpp6c | EIF3S4 (9), FTL (4), SNX9 (4), THOP1, FLJ14502, DNLC2A, MT2P1, BM806939, RPS20, BRG1, DYN2, AATF, MacGAP |
| Rrp44 | — |
| PinC [1-182] | AD-003 (2), ZNF151 |
| PinC + CSP [1-387] | — |
| RNase II + RNB [388-958] | — |
| Ski2b | — |
| helicaseb [1-708] | — |
| [709-1246] | SmB (5), AL517440 (2), FLJ34136, GALNT13, BF248318, U80752, WDR1, CDC2L2 |
| Lsm1 | VPS11 (20), Lsm2 (12), NARS (9), PSMB8 (7), Lsm3 (5), PSMB5 (2), FLJ20254 (2), UXT, N4BP1, GRHPR, HSPC1, DKFZP564J0123, AF090094, AW27564, AW974116, AW975586, KIAA0179 |
| Lsm2e | Lsm8 (88), Lsm3 (75), SmE (5), Znf408 (3), DNAJL2 (2), Lsm7, SmD2 |
| Lsm3 | Lsm2 (51), Lsm8 (36), Lsm10 (21), SmD3 (14), SmE (8), Lsm7 (8), XPC, BC009518 |
| Lsm4 | Lsm8 (32), Lsm7 (27), FLJ13289 (18), SmE (11), Nmi (4), PEPP-2 |
| Lsm5 | Lsm7 (75), SmF (13), SmE, Lsm3 |
| Lsm6 | Lsm5 (40), SmD2 (37), Lsm7 (32), Lsm3 (7), MCRS1 (4), POLD2 |
| Lsm7 | Lsm5 (61), SmD3 (3), Lsm4 (3), RAB7L1 (2), NPY1R, MYH13 |
| Lsm8c | Lsm2 (17), NY-REN-45 (8), FLJ23534 (8), LSM4 (5), TALDO1 (4), SmD1 (3), PDCD2 (3), DKFZp565A176 (3), PGD (2), ASK (2), FLJ23469 (2), RPL24, SMBP2, PNUTL1, MTHFR, DKFZP434F091, MGC10966, GTF21, ITSN2, FLJ11280, DKFZp434K0621, IFNLR1 |
| Dcp2 | POLA2 (7), NAGK, PSMB1 |
| Dcp28 (Δ exons 8 and 9) | RCD-8 (7) |
| Dcp1Ab | — |
| Dcp1Bb | — |
| DcpS | — |
| Xrn1/Sep1 | BAT3 (2), LDHA (2), PABPC4, BC001200, ALDOA, MGC13198, AP1G2, STAM2A, CHD4, |
| Exonuclease [1-300] | SMARCD2, CCT5, GRIM19, FLJ10359, FLJ21347, AI821928 |
| [578-1470] | — |
| RBD [1440-1707] | hnRNPA1 (2), KIAA0261 (2), DPP2 (2) and many others |
| Xrn2/Rat1 | PSMA3 (6), EIF3S6IP, AJ420488, AW977134 |
| Exonuclease [1-280] | EIF6 (2), EIF5A, DKFZP434J214, COMT, MOCS3, TOLLIP, TARDBP |
| Dom3d | AI580336 |
| PARN | DKFZp434N0650 (12) |
| TTPb | — |
| Auf1/hnRNPDc (variant 3) | SF2p32 (30), Rrp41, Lsm5 plus many others |
| HuR | — |
| Upf1 [1-415] | — |
| Helicase [416-701] | — |
| [702-1119] | HIRA (2), BC040175, N21259, PLEKHB2, FLJ10631, ACAS2, BAT5, PEPP2, KIAA0460, GNPTAG |
| Upf2 [1-167] | — |
| MIF4G1 + 2 [166-758] | — |
| [757-1272]c | RPS15 (12), RPL5 (5), REA (3), MAP1A (3), RPS25 (2), ART4 (2), UPF3B + many others |
| Upf3A (variant2) | GSK3B (2), BM310104 (2), IFITM2 |
| Upf3B | MCRS1, FLJ20343 |
| Znf408
|
RGS19IP1 (6), RIL (2), NOH61 (2), Lsm2, Znf408, NOA36, DKFZP434G156, BG403593, BQ069192, MIF, TOP3B, CAF1B
|
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aFor descriptions of baits, see Table 1.
Previously published interactions are in bold, and interactions detected between the orthologous yeast or Drosophila proteins in genome-wide data sets are underlined.
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↵b Self-active bait.
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↵c Weakly self-active bait (— His selection).
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↵d The number of colonies isolated for each bait is indicated in parentheses when greater than 1. Singly isolated preys are not listed for baits that isolated >20 different preys. A few interactions with extracellular proteins or those known to localize to the secretory pathway or mitochondria are not listed, because they are unlikely to represent physiological interactions. A full list of interacting preys, accessions, and descriptions are available from http://www.hgmp.mrc.ac.uk/Research/RNA and as Supplemental material.
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↵e Lsm2 and Dom3 library screen interactions were previously reported (Lehner et al. 2004).
