Protein–protein interactions detected between proteins predicted to function in human RNA decay. (A) Each Y2H interaction (selected on media lacking Ade or His) is indicated as an arrow from bait to prey. The amino acid residues included in protein fragments are indicated in brackets. Very weak interactions are not shown. The figure was drawn using BioLayout (http://maine.ebi.ac.uk:8000/services/biolayout/; Enright and Ouzounis 2001). (B) The interactions of the Upf proteins suggest how both nuclear and cytoplasmic 5′→3′ and 3′→5′ decay factors may be recruited during NMD. Y2H interactions are shown as arrows, proteins required for NMD are indicated by *, and proteins that coprecipitate from cell extracts are underlined (Lejeune et al. 2003).
