Figure 1
Subunit interactions of the human exosome. (A) Y2H interactions detected on stringent (–Ade) and less-stringent (–His) selection are indicated. The amino acid residues included in protein fragments are shown in brackets. (*) Self-active bait. (B) Stringent Y2H interactions are superimposed on a model of the exosome. The Rrp41-PM/Scl-75 interaction was only detected by library screening (see Table 2). Interactions previously detected by mammalian two-hybrid (M2H) and between the homologous yeast proteins are indicated. PH-domain subunits are blue, S1-domain subunits are red, and other subunits are yellow.
