Local Definition of Ty1 Target Preference by Long Terminal Repeats and Clustered tRNA Genes

Table 6.

Optimized PCR Conditions


Primer 1

Primer 2

Annealing temp

Cycle number
Ty1: JB2930 tGly: JB2821 60 30
Ty1: JB2930 tThr: JB3084 54 30
Ty1: JB2930 tG(GCC)D1: JB6071 61 30
Ty1: JB3361 tGly: JB2821 60 30
Ty1: JB3361 tThr: JB3084 60 32
Actin: JB4307
Actin: JB4312
54
20

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  1. Genome Res. 14: 1232-1247

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