Figure 5
Theoretical and measured maps of λ phage DNA obtained with S-1TMR, S-2TMR, S-1Alx, and S-2Alx bisPNA tags (Table 1). These tags all have the same sequence motif (amino terminus)TTTCTCTT(carboxyl terminus), which recognizes the target (5′)AAGAGAAA(3′). (A) Theoretical map obtained for this motif. (B–E) Experimental maps obtained with S-1TMR, S-2TMR, S-1Alx, and S-2Alx bisPNA tags, respectively. The data sets used to obtain the maps B, C, D, and E included 2279, 2275, 145, and 943 total individual molecular traces, respectively. See Figure 4 caption for details.
