Workflow of phenotype classification system. The mix of targeted DNA (e.g., subcellular clones–GFP fusion), gelatin, and transfection reagents are prepared in microwells and spotted in arrays into chamber slides by a DNA-spotting robot. Cultured cells are added to the chamber slides and transfected with the DNA on the arrayed spots. After 10 h incubation at 37°C the fluorescence signal of the expressed GFP signal can be visualized. On the automatic scanning platform a motorized stage and motorized z-stepper perform the scanning of the live cells. The control of the illumination and the automatic calculation of the integration time allow an automatic acquisition of cell images. Cells with GFP-signals are captured and image features are extracted. These features serve as input for training of the automated classification system.
