Figure 3
(A) NiCl2 titration for Tetr negative selection marker. Bacterial cells with vectors that either contained the CAP resistance gene (control vector) or both the CAPr and Tetr (Tet lox-destination vector) were plated on CAP containing the indicated concentration of NiCl2. The log units of surviving cells are shown on the y-axis. (B) Sucrose titration for sacB negative selection marker. Bacterial cells with vectors that contained either the SacB lox-destination vector (CAPr and sacB) or a control vector (CAPr only) were plated on CAP containing the indicated percentage of sucrose. The log units of surviving cells are shown on the y-axis.
