Systematic Insertional Mutagenesis of a Streptomycete Genome: A Link Between Osmoadaptation and Antibiotic Production

Table 2.

Oligonucleotides Used. The Nonhomologous Tail in the S1 Mapping Primer S1-04nht Is Underlined


Tn5062 linker oligonucleotides
VC1 5′-GATCTGAATTCGGATCCTAATTAATTAATCTAGAAAGGAGGTGATCA
VC2 5′-TATGATCACCTCCTTTCTAGATTAATTAATTAGGATCCGAATTCA
VC3 5′-TATGGACGGAGCTCGGCCGCTTAAGGTACCGAATTCC
VC4
5′-TCGAGGAATTCGGTACCTTAAGCGGCCGAGCTCCGTCCA
Tn5062 sequencing primers
EZR1 5′-ATGCGCTCCATCAAGAAGAG
EZL2
5′-TCCAGCTCGACCAGGATG
IPCR primers
Tsr1 5′-GCAAGGGGAAGTCGTCGCTCTCTGG
Tsr2 5′-CGGCGTGGGGACGTCGTCGTTCTCG
Tsr3 5′-GGATTTGCGATGGTGTCCAACTCAG
Tsr4 5′-GGACGTCGAGCCCCTCATGCACAGC
Tsr5 5′-GGAGAGAACGACGGGAAGGGAGAAG
Tsr6 5′-GACCTCGATTCGTCAGTGATGATCC
Tsr7 5′-CGGATTGTCCCCGAGTTCCTTCACG
Tsr8
5′-GCGTCTTCCGCCTCGGTTTCCATCC
S1 mapping primers
S1-04nht 5′-GCGCAAGCTTCCCGAAGCCGTGTAGAAGTA
S1-04r
5′-AGTGCTTTGAGCGCTTCCT

This Article

  1. Genome Res. 14: 893-900

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