Table 1.
Bacterial Strains and Plasmids
|
Strain or plasmid |
Characteristics |
Reference or source |
|---|---|---|
| S. coelicolor M145 | prototrophic, SCP1- SCP2- PgI+ | Kieser et al. 2000 |
| S. lividans 1326 | SLP2+ SLP3+ | Kieser et al. 2000 |
| S. lividans TK64 | pro-2 str-6 SLP2- SLP3- | Kieser et al. 2000 |
| S. lividans X0236 | osaB::Tn5493 | This work |
| S. coelicolor AB001 | osaB::Tn5062 | This work |
| S. lividans AB101 | osaB::Tn5062 | This work |
| S. coelicolor AB201 | osaA::Tn5062 | This work |
| E. coli JM109 | F′ traD36 proA+B+ lac1q Δ(lacZ)M15/Δ(lac-proAB) glnV44 e14- gyrA96 recA1 relA1 endA1 thi hsdR17 | Yanisch-Perron et al. 1985 |
| E. coli ET12567 (pUZ8002) | dam13::Tn9 dcm6 hsdM hsdR recF143 16 zjj201::Tn10 galK2 galT22 ara14 lacY1 xyl5 leuB6 thi1 tonA31 rpsL136 hisG4 tsx78 mtli glnV44, containing the nontransmissible oriT mobilizing plasmid, pUZ8002 | |
| SC7C7 | Supercos-1 containing chromosomal DNA from S. coelicolor; bla kan | Redenbach et al. 1996 |
| pET26B+ | E. coli cloning vector; kan | Novagen |
| pALTER1 | E. coli cloning vector; bla tet | Promega |
| pEGFP-N1 | E. coli mammalian eGFP shuttle vector; kan | Clontech |
| pHP45Ωacc | E. coli vector; aac3(IV) | Blondelet-Rouault et al. 1997 |
| pMOD〈MCS〉 | E. coli vector with Tn5 inverted repeats; bla | Epicentre |
| pIJ8660 | integrative vector for Steptomyces; oriT (RK2), aac3(IV) | Sun et al. 1999 |
| pFP11 | pALTER1 containing eGFP | This work |
| pFP12 | pFP11, eGFP with 5′ Ndel site | This work |
| pVC101 | pET26B+ containing three-frame translational stop | This work |
| pVC102 | pVC101 containing streptomycete rbs | This work |
| pVC107 | pVC102 containing eGFP | This work |
| pQM501 | pALTER1 containing aac3(IV) | This work |
| pQM504 | pMOD〈MCS〉 containing aac3(IV) | This work |
| pQM5052 | pQM504 containing oriT(RK2) | This work |
| pQM5062 | pQM5052 containing eGFP; Tn5062 | This work |
| pUC18 | E. coli cloning vector; bla | Yanisch-Perron et al. 1985 |
| pD81 | pALTER1 containing osaB, SCO5750, aac3(IV), tet | This work |
| pD04 | pUC18 containing osaB | This work |
| pAD04 | pD04 ligated to pALTER1 | This work |
| pPM927 | integrative vector for Streptomyces; oriT(RK2) int attP (pSAM2) aadA, tsr | Smokvina et al. 1990 |
| pPM04
|
pPM927 containing osaB
|
This work
|
