Figure 4
Transcription of osaB. (A) Fluorescence and phase-contrast images of substrate mycelia after 24 h growth of S. coelicolor AB001 on R2YE medium (i) with or (ii) without 10.3% sucrose (Bar, 10 μm). (B) High resolution SI nuclease mapping of the osaB transcription start site. (i) RNA was isolated at the time points indicated from cultures of S. coelicolor M145 grown on R2YE medium containing 10.3% sucrose. (ii) The corresponding nucleotide sequence of the promoter region of osaB, indicating -35 and -10 sequences, transcriptional start (arrow), the probable ribosome-binding site (rbs), and translational start (TS).
