Table 2.
DASL Performance as a Function of RNA Degradation Level
|
Duration of treatment at 95°C |
0 min |
5 min |
10 min |
20 min |
40 min |
60 min |
|---|---|---|---|---|---|---|
| % RNA detectable by RT—PCR | 100 | 53.02 | 22.90 | 5.47 | 0.75 | 0.13 |
| Correlation between technical replicates, R2 | 1.00 | 1.00 | 1.00 | 0.99 | 0.98 | 0.94 |
| Correlation with undegraded RNA, R2 | 1.00 | 0.91 | 0.83 | 0.77 | 0.71 | 0.61 |
| % of genes detected, compared to undegraded RNA
|
100
|
99
|
98
|
97
|
94
|
88
|
