Table 1.
DASL Performance as a Function of RNA Input
|
RNA input |
100 ng |
75 ng |
50 ng |
25 ng |
10 ng |
5 ng |
|---|---|---|---|---|---|---|
| % genes “on” | 78 | 76 | 74 | 73 | 70 | 70 |
| % genes with <2-fold difference detectable | 95 | 80 | 84 | 82 | 59 | 41 |
| Correlation (R2) compared to 100 ng
|
1.000
|
0.986
|
0.983
|
0.970
|
0.946
|
0.918
|
-
Varying levels of total RNAs from A-20 and EL-4 cells were input to DASL assays. Values shown for detection and reproducibility are the average of those for each cell line. The two cell lines were compared for the proportion of genes differentially expressed as a function of input.
