(A) Decoding images from eight sequential stages (numbered). Each image is a false-color composite of the FAM and Cy3 grayscale images from each stage. A small region (<0.2%) of a single array is shown. The circled bead is one of up to ∼4.8 million in a single 96-array matrix. It has the code 11012202. A total of 1728 images, each ∼5 Mb in size and containing ∼1.7 million pixels, are collected in order to decode an array matrix. (B) Histogram of bead intensities for a single stage. The low intensity peak includes beads in the OFF state and empty wells. The higher intensity peak includes beads in the ON state. (C) The eight-stage intensity profile of a typical individual bead (code = 02212110) in the FAM and Cy3 channels (∼100 counts of intensity are from camera DC offset).
