Table 1.
Rattus norvegicus BAC Libraries
|
Library |
RPCI-32 |
CHORI-230 |
||||
|---|---|---|---|---|---|---|
| Segment |
1 |
2 |
1 |
2 |
||
| Cloning vector | pBACe3.6 | pBACe3.6 | pTARBAC2.1 | pTARBAC2.1 | ||
| DNA source | Male spleen | Male spleen | Female brain | Female liver | ||
| Restriction enzyme | EcoRI | EcoRI | EcoRI | MboI | ||
| Plate range | 1-288 | 289-624 | 1-240 | 241-528 | ||
| Total 384-well dishes | 288 | 336 | 240 | 288 | ||
| Empty wells (%) | 1244 (1.1%) | 1559 (1.2%) | 1742 (1.9%) | 1677 (1.5%) | ||
| Noninsert clones (%) | 4/159 (2.5%) | 7/156 (4.5%) | (1.1%) | 26/382 (6.8%) | ||
| Recombinant clones | ∼106,000 | ∼122,000 | ∼89,000 | ∼102,000 | ||
| Insert size (Average) | 147 kb | 153 kb | 214 kb | 168 kb | ||
| Standard deviation | 35 kb | 29 kb | 27 kb | 53 kb | ||
| Library redundancy
|
5.2-fold
|
6.2-fold
|
6.4-fold
|
5.5-fold
|
||
-
The “Recombinant clones” column represents total wells after empty wells and noninsert clones were subtracted in each segment. The library redundancy was estimated using 3000 Mb as the genome size.
