Figure 1
Construction of pTARBAC1.3 and pTARBAC2.1 vectors. Two BstXI (position 5884 and 9327) and one ApaLI (position 5164) sites (see arrows) were eliminated from the pTARBAC1 vector to create the pTARBAC1.3 vector. The pTARBAC2.1 vector was generated from pTARBAC1.3 by replacing the two BamHI-EcoRI fragments with synthetic adapters to regenerate the EcoRI sites while eliminating the BamHI sites.
