Table 2.
Comparison of Gel Analysis Assay for RT-PCR Success to Sequence Analysis Assay
|
Sequence analysis |
One right size band |
Multiple bands ≥ right size |
No right size band |
Total |
|---|---|---|---|---|
| Hit | 65 | 35 | 54 | 154 |
| Non-hit
|
5
|
38
|
166
|
209
|
| Total
|
70
|
73
|
220
|
363
|
-
Products that gave high-quality sequence with a clear spliced alignment to the genome in the target region are classified as hits; all others are classified as non-hits.
