Identification of Rat Genes by TWINSCAN Gene Prediction, RT–PCR, and Direct Sequencing

Table 1.

Summary of RT-PCR Success Rates


Target type

Number of targets

Number (%) successful
Single intron 444 260 (59%)
Most of ORF (TE primers) 152 106 (70%)
Complete ORF (UTR primers)
136
46 (34%)
  • Targets for TE and UTR primers were selected from the single intron successes.

This Article

  1. Genome Res. 14: 665-671

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