Figure 1
A TWINSCAN prediction (green, subsequently identified as rat aspartylglucosaminidase) in which sequences from the single intron (purple), terminal exon (red), and UTR (blue) primer pairs all yielded spliced alignments. Primers are shown as tall pink blocks at the same level as the sequence they yielded (the left single-intron primer did not yield high-quality sequence). TWINSCAN makes use of the blocks of alignment from the human genome (black) and the mismatches and gaps within the blocks (red) in predicting the most likely gene structure.
