Gels used as part of RT-PCR validation of predicted introns. RT-PCR was used to validate one intron from 48 predicted novel human genes and their simultaneously predicted rat orthologs. The gels contain the RT-PCR sequenced products from human (A) and rat (B). Each column in the gel contains the sequenced product for one intron tested, with the same columns in the human and rat gels containing products from orthologous predictions. The leftmost and rightmost columns contain the ladders used to determine the lengths of the sequenced products. The second column contains a positive control (RT-PCR of an exon-pair from actin), and the third column contains a negative control (no reverse transcriptase used).
