Tracking the Evolution of the SARS Coronavirus Using High-Throughput, High-Density Resequencing Arrays

Table 1.

Call Rate and Accuracy of SARS Resequencing Array


Array sequence

Discordant calls

Ambiguous calls (Ns)

Call rate

Accuracy
SIN2500 3 495 98.33% 99.989%
SIN2677 4 179 99.40% 99.986%
SIN2679 0 138 99.53% 100%
SIN2748 2 223 99.22% 99.993%
Vero isolate 1 1 230 99.02% 99.997%
Vero isolate 2 1 328 98.89% 99.997%
Vero isolate 3 4 183 99.38% 99.986%
Vero isolate 4 0 198 99.33% 100%
Vero isolate 5 0 218 99.36% 100%
Vero isolate 6 0 210 99.29% 100%
Vero isolate 7 0 307 98.96% 100%
Vero isolate 8 0 220 99.26% 100%
Tissue 1-1 1 227 99.24% 99.993%
Tissue 1-2 1 982 96.69% 99.997%
Tissue 1-3 2 120 99.60% 99.997%
Tissue 2
0
278
99.07%
100%
  • Discordant calls differ between array and ACS. Ambiguous calls refer to bases that lack sufficient information for high-confidence base assignment. Call rate is the percentage of genome sequence with high-confidence base calls. Accuracy is the percentage of correctly called bases (as determined by ACS) over the total number of bases called (excluding ambiguous calls). These results are based on duplicate hybridizations. Tissue 1 was hybridized on three pairs of arrays. The data for tissues 3 and 4 are not shown as ACS sequence is not available.

This Article

  1. Genome Res. 14: 398-405

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