Relationship Between Sex Bias in Gene Expression in Normal Individuals and Misregulation in Hybrids
|
Misregulation in the hybrid |
% female-biased |
Sex bias in gene expression % male-biased |
% nonsex-biased |
|---|---|---|---|
| Underexpression | 84.5 (+45)/93.9 (+54.4) | 4.7 (-19.2)/1.8 (-22) | 10.8 (-25.9)/4.3 (-32.3) |
| Overexpression
|
1.1 (-38.4)/0.3 (-39.1)
|
54.6 (+30.9)/58.3 (+34.5)
|
44.3 (+7.6)/41.3 (+4.6)
|
-
Before the slash, percentage of genes regardless of the level of misregulation in the hybrid in relation to the parental species; after the slash, percentage of misregulated genes with a difference in gene expression >2. In parenthesis, difference between the observed and the expected percentage of genes in each category according to its reported representation on the array (Ranz et al. 2003): female-biased (39.5%); male-biased (23.8%); nonsex-biased (36.7%). Classification in one particular category involves that the same tendency was found for both D. melanogaster and D. simulans. Differences in expression between the hybrid and the parental species must be significant at P < 0.01, both with BAGEL and with the mixed-model ANOVA, for a gene to be included in the analysis. Also, only genes from Ranz et al. (2003) regarded here as consistent (see Methods) are included in the analysis. Number of underexpressed genes = 1194; number of overexpressed genes = 948; number of underexpressed genes above twofold = 603; number of overexpressed genes above twofold = 383.
