Figure 1
Set of competitive hybridizations onto cDNA microarrays performed to compare the expression profile among D. melanogaster, D. simulans, and hybrid females. Twenty-one hybridizations were carried out with mRNA from whole flies (solid arrows) and 12 with head-specific amplified mRNA (dashed arrows). Arrowhead, sample labeled with Cy5; base of arrow, sample labeled with Cy3. Each comparison was replicated six times for whole-fly mRNA and four times for head mRNA. Three self-self hybridizations were also carried out for the whole-body experiment. The resulting experimental design was balanced for dyes and samples.
