Example of the BAC/BIBAC contigs of the soybean physical map. This contig (ctg16) was anchored to the molecular linkage group MLG F of the soybean genetic map by the SSRmarker Satt343f (Cregan et al. 1999a; http://soybase.agron.iastate.edu/). The highlighted clones were the positive clones of the SSRmarker. The contig contains 120 BAC and BIBAC clones and 651 unique bands, estimated to span ∼2643 kb in physical length. The clones prefixed with “IS” or “UM” were from the Williams 82 and Faribault BAC libraries, respectively; and the clones prefixed with “E,” “B,” or “H” were from the Forrest EcoRI BAC, BamHI, and HindIII BIBAC libraries, respectively.
