Table 2.
Observed and Expected Numbers of Nucleotide Substitutions Along With Estimates for Constraint in Noncoding DNA Sequences of Three Categories, Calculated by the Two Lineage Approach Using Four-Fold Sites of Homologous Genes from D. simulans and D. melanogaster as a Standard
|
|
|
|
|
Substitutions per locus |
|
||
|---|---|---|---|---|---|---|---|
| DNA category
|
Data set
|
Number of loci
|
Base pairs per locus
|
Observed (SEM)
|
Expected (SEM)
|
Constraint (SEM)
|
|
| Intronic | Complete | 91 | 228 | 16.23 (1.69) | 14.86 (1.56) | –0.094 (0.059) | |
| Intronic | Splice sequences omitted | 91 | 190 | 13.72 (1.63) | 11.75 (1.45) | –0.17 (0.069) | |
| 5′ intergenic | bp 1–100 | 77 | 99 | 5.43 (0.36) | 8.29 (0.46) | 0.34 (0.053) | |
| bp 101–200 | 73 | 98 | 5.58 (0.45) | 7.97 (0.46) | 0.30 (0.056) | ||
| bp 201–300 | 69 | 98 | 6.51 (0.53) | 7.82 (0.49) | 0.16 (0.079) | ||
| bp 301–500 | 66 | 188 | 13.37 (1.06) | 13.86 (0.86) | 0.03 (0.088) | ||
| 3′ intergenic | bp 1–100 | 42 | 95 | 5.78 (0.69) | 7.29 (0.54) | 0.21 (0.086) | |
| bp 101–200 | 31 | 88 | 5.35 (0.77) | 6.58 (0.83) | 0.17 (0.14) | ||
| bp 201–300 | 21 | 92 | 5.91 (0.79) | 6.65 (1.06) | 0.093 (0.17) | ||
|
|
bp 301–500
|
18
|
150
|
7.10 (1.30)
|
11.60 (2.13)
|
0.36 (0.19)
|
|
