Figure 4
Monoallelically methylated CGIs and their nearest neighbor genes. (A) The positions of CGI #112, #59, and #130 are depicted with their nearest neighbor genes. (B) The allelic expression status of DSCR3 wasexamined by direct sequencing of RT-PCR productsderived from PBLsof an A/T heterozygote. (C) An RT-PCR-RFLP assay was developed for SLC19A1 using HhaI, and applied to cDNAsfrom PBLsof three individuals(left, A/G heterozygote; middle, A/A homozygote; right, G/G homozygote). Note that the primers used in this assay simultaneously amplify two splicing isoforms, which are detected astwo distinct bandsin the gel.
