Figure 3
Expression of genes methylated in their promoters or 5′-UTRs. RT-PCR was performed using total RNA from various human tissues (1, bone marrow; 2, adrenal gland; 3, thymus; 4, prostate; 5, trachea; 6, thyroid; 7, spleen; 8, small intestine; 9, colon; 10, uterus; 11, placenta; 12, testis; 13, heart; 14, skeletal muscle; 15, liver; 16, lung; 17, kidney; 18, whole brain; 19, salivary gland; 20, peripheral blood cells). The products were resolved by polyacrylamide gel electrophoresis, stained with SYBR Green, and visualized by UV illumination.
