Figure 3
Propagation of BACs with GAP6 sequence in E. coli at 37°C and 30°C. BAC DNA was isolated from three E. coli subclones of the clones #1, #2, #3, and #4 carrying GAP6, digested with NotI, and separated by gel electrophoresis. Deleted/rearranged isolates of BACs grown at 37°C were partially stabilized by growing at 30°C. M1, 1-kb ladder marker; M2, 48-kb ladder marker.
