Figure 6
The up1 homology of the ctgf gene mediates midline expression in the zebrafish. (A) Comparison of the up1 homology from the Takifugu and the zebrafish ctgf gene. A putative TATA box and the end of the zebrafish ctgf cDNA are indicated. Underlined sequences represent three regions (M1 to M3) partially conserved in the floor plate/notochord enhancers ar-A, ar-B, and ar-C of shh and the FPE of netrin1. H indicates a putative FoxA2/Axial/HNF3β binding site. (B) Transient transgenic embryo expressing GFP under control of the up1 region from the tk:gfp cassette. (C) Transient transgenic embryo expressing GFP from the ctgf:gfp transgene. (f) Floor plate; (n) notochord.
