Figure 1
Strategy to enrich for midline genes. Transgenic embryos carrying the -2.2shh:gfpABC (left) or the -8.4ngn1:gfp transgene (right) were dissociated, and GFP-positive cells were sorted with a fluorescence-activated cell sorter. cDNA was prepared from GFP-positive cells and subtracted crosswise before analysis of subclones and microarray hybridization. The complex probe S is enriched for midline specific genes, whereas the reverse subtraction (probe N) serves as a hybridization control.
