Table 1.
Identified mutations in E. coli-K12 MG1655 clones underlying adaptive evolution on glycerol
|
Gene (protein) |
Position |
Mutation |
Affected amino acid |
Mutant clones |
|---|---|---|---|---|
| glpK (glycerol kinase) | 113 | A → C | Q38P | GlyE |
| 160 | T → G | W54G | MG, MGA | |
| 184 | G → T | V62L | GlyC | |
| 218 | A → C | D73A | GlyA, GlyB | |
| 218 | A → T | D73V | PG | |
| 692 | G → A | G231D | G1, GP, GPA | |
| 705 | Ins. AAAGGCGGC | Ins. 235GGK | G2 | |
| aldB (aldehyde dehydrogenase B) | 36 | C → A | P12P | G1, GP, GPA |
| tdcR (threonine dehydratase activator) | +4 | C → A | (L + 2I) | G1-A |
| rpoS (σ factor 38)
|
739
|
G → T
|
E247stop
|
G1, GP, GPA
|
