High-throughput mutation detection underlying adaptive evolution of Escherichia coli-K12

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Figure 4.
Figure 4.

Mutation detection in the glycerol kinase gene (glpK) by base-specific cleavage and MALDI-TOF MS. Discriminating mass signals in the T-cleavage reaction of the reverse RNA transcript (highlighted in boxes) at 2541.6 Da, 2581.6 Da, 2886.8 Da, 3256.0 Da, and 3272.0 Da allow for unambiguous identification of the substitution T→G at position 160, G→T at position 184, A→C at position 218, and A→T at position 218.

This Article

  1. Genome Res. 14: 2495-2502

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