Table 2.

Validation of MIR expression in the five human tissues by the mirMASA method

	Blank^a	Brain			Match^d			Liver			Match			Thymus			M^e	Testes
Sample	Signal^b	Signal	Ratio^c	NB	CH		Ratio	NB	CH		Ratio	CH		Ratio	CH		Ratio	CH
Let-7b	83	5102	62.5	+	+	1436	17.3	+	+	3342	40.3	+	3292	39.7	+	1175	14.2	+
mir-20	67	554	8.3	–	+	515	7.7	–	+	1193	17.9	+	970	14.6	+	751	11.3	+
mir-127	89	179	2.0	+	–	128	1.4	+	–	102	1.2	–	125	1.4	–	127	1.4	–
mir-129	84	118	1.4	+	–	96	1.1	+	+	93	1.1	+	104	1.2	–	120	1.4	+
mir-137	73	85	1.2	–	+	81	1.1	+	+	68	0.9	+	63	0.9	+	75	1.0	+
mir-141	76	115	1.5	+	+	102	1.3	+	+	154	2.0	+	165	2.2	+	664	8.8	+
mir-199a	103	187	1.8	–	+	454	4.4	+	+	979	9.5	+	1671	16.2	+	1552	15.0	+
mir-203	56	78	1.4	–	+	81	1.4	–	+	99	1.8	+	73	1.3	+	79	1.4	+
5sRNA-1	111	3630	32.8			4059	36.7			3450	31.2		3758	33.9		4256	38.4
5sRNA-2	81	3016	37.2			3180	39.3			2878	35.5		3078	38.0		3317	41.0

↵a Signal obtained from incubation of the capture and detection oligo's without RNA
↵b The expression of each MIR in each tissue was measured in duplicates. Difference between duplicates was ∼5%–10%. The average of the duplicates is presented
↵c The ratio between the signal value and the Blank value
↵d Match between the mirMASA, Northern blot (NB), and DNA chip (CH) data. (+) A match (–) no match
↵e M is short for match. For the thymus, testes, and placenta, no Northern blot data is available, and comparison was done only to DNA chip data

MicroRNA expression detected by oligonucleotide microarrays: System establishment and expression profiling in human tissues